Fmoc-L-glutamic acid-gamma-benzyl ester (CAS# 123639-61-2)
Fluorene methoxycarbonyl-L-glutamic acid-Γ-benzyl wani nau'in halitta ne da ake amfani da shi a cikin haɗin peptide a cikin haɓakaccen lokaci mai ƙarfi. Yanayinsa:
- Bayyanar: Fari zuwa kodadde rawaya m
- Solubility: Fmoc-L-Glu(OtBu) -OH yana da mai kyau solubility tsakanin na kowa Organic kaushi.
Babban amfani da Fmoc-L-Glu (OtBu) -OH shine azaman ƙungiyar karewa a cikin haɗin peptide. Lokacin haɗa sarƙoƙin peptide, Fmoc-L-Glu (OtBu) -OH yana ɗaure ga amino acid, yana kare ayyukansu daga halayen da ba takamaiman ba tare da sauran masu amsawa. Bayan an gama amsawa, za a iya cire Fmoc-L-Glu(OtBu) -OH ta hanyar cire rukunin kariya don dawo da ayyukan amino acid.
Shirye-shiryen Fmoc-L-Glu(OtBu) -OH yana da ɗan rikitarwa kuma gabaɗaya yana buƙatar amfani da jerin matakan haɗaɗɗun kwayoyin halitta. Glutamic acid yana amsawa tare da bromoacetate don samun ethyl glutamate. Sa'an nan, ethyl glutamate yana amsawa tare da barasa benzyl don samar da ethyl glutamate benzyl barasa ester. An mayar da Ethyl glutamate benzyl barasa ester tare da Fmoc-Cl don samar da samfurin da aka yi niyya Fmoc-L-Glu(OtBu) -OH.
Bayanin Tsaro: Fmoc-L-Glu(OtBu) -OH magani ne na dakin gwaje-gwaje kuma yana buƙatar amfani da shi ƙarƙashin aikin dakin gwaje-gwaje mai aminci. Bi ƙa'idodin aminci na ɗakin gwaje-gwaje na gabaɗaya, gami da sanya kayan kariya na sirri (misali, safar hannu na lab, tabarau, da sauransu), guje wa hulɗar fata da shakar numfashi, da aiki a cikin dakin gwaje-gwaje mai isasshen iska. Ya kamata a adana mahallin a cikin akwati mai hana iska, nesa da kunnawa da oxidants.
