Isopropyl-beta-D-thiogalactopyranoside (CAS#367-93-1)
Hadari da Tsaro
| Lambobin haɗari | R19 - Zai iya samar da peroxides masu fashewa R40 - Shaida mai iyaka na tasirin cutar sankara R66 - Maimaita bayyanarwa na iya haifar da bushewar fata ko tsagewa R36 / 37/38 - Haushi ga idanu, tsarin numfashi da fata. |
| Bayanin Tsaro | S23 - Kar a shaka tururi. S24/25 - Guji hulɗa da fata da idanu. S36/37 - Sanya tufafin kariya da safar hannu masu dacewa. S22 - Kada ku shaka kura. S36 / 37/39 - Sanya tufafin kariya masu dacewa, safar hannu da kariya / ido / fuska. S27 – Cire duk gurbatattun tufafi nan da nan. S26 - Idan ana hulɗa da idanu, kurkura nan da nan da ruwa mai yawa kuma ku nemi shawarar likita. |
| WGK Jamus | 3 |
| FLUKA BRAND F CODES | 10 |
| Farashin TSCA | Ee |
| HS Code | 29389090 |
Gabatarwa
IPTG abu ne mai haifar da ayyuka na β-galactosidase. Dangane da wannan sifa, lokacin da vector DNA na pUC jerin (ko wasu vector DNA tare da lacZ gene) aka canza tare da lacZ shafe Kwayoyin a matsayin mai watsa shiri, ko lokacin da vector DNA na M13 phage aka canza, idan X-gal da IPTG an kara. zuwa matsakaicin farantin karfe, saboda α-complementarity na β-galactosidase, ana iya zaɓar recombinant ta hanyar sauƙi gwargwadon ko mazaunan fararen fata (ko plaques) suna bayyana. Bugu da kari, ana iya amfani da shi azaman inducer magana don maganganun maganganu tare da masu tallata kamar lac ko tac. Mai narkewa a cikin ruwa, methanol, ethanol, mai narkewa a cikin acetone, chloroform, mai narkewa a cikin ether. Yana da inducer na β-galactosidase da β-galactosidase. Ba a sanya shi ta hanyar β-galactoside. Yana da maganin substrate na thiogalactosyltransferase. Formulated: IPTG yana narkar da cikin ruwa, sa'an nan kuma haifuwa don shirya wani ajiya bayani (0 · 1M). Mahimmancin IPTG na ƙarshe a cikin farantin mai nuna alama yakamata ya zama 0 · 2mM.
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