N(alpha) -fmoc-N(epsilon)-(2-chloro-Z) -L-lysine(CAS# 133970-31-7)

Fmoc-(2-chlorobenzyloxycarbonyl) lysine asalin amino acid ne da aka saba amfani dashi tare da kaddarorin masu zuwa:1. Bayyanar: farin crystalline foda;
2. tsarin kwayoyin halitta: C26H24ClNO5;
3. Nauyin kwayoyin halitta: 459.92g / mol;
4. Solubility: Mai narkewa a cikin kwayoyin halitta irin su dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), dichloromethane, da dai sauransu, wanda ba a iya narkewa a cikin ruwa;
5. Matsayin narkewa: game da 170-175 ° C. Amfani na farko na Fmoc- (2-chlorobenzyloxycarbonyl) lysine shine a matsayin ƙungiya mai karewa da kunnawa a cikin haɗin polypeptides. Za a iya kunna ƙungiyar carboxyl ɗin ta don samar da ester, wanda daga nan sai ya sami amsawa tare da ragowar amino acid don haɗa sarkar polypeptide. Za a iya cire ƙungiyar Fmoc cikin sauƙi bayan an gama amsawa don fallasa ƙaƙƙarfan sassan amino.

Hanyar shirya Fmoc- (2-chlorobenzyloxycarbonyl) lysine gabaɗaya ya ƙunshi matakai masu zuwa:

1. amsa lysine tare da N-hydroxybutyrimide (Pbf) don gabatar da ƙungiyar karewa;
2. amsawar lysine-Pbf tare da 2-chlorobenzyl barasa don samar da Fmoc- (2-chlorobenzyloxycarbonyl) lysine;
3. Ana fitar da samfurin tare da mai dacewa mai dacewa kuma an tsarkake shi ta hanyar crystallization don samun samfurin mai tsabta.

Game da bayanin aminci, Fmoc- (2-chlorobenzyloxycarbonyl) lysine reagent sinadari ne kuma yakamata a ɗauki matakan tsaro masu dacewa. Ya kamata a sa kayan kariya da suka dace, kamar safofin hannu na lab, tabarau, da tufafin lab, yayin gwajin. Ka guji shakar foda ko mafita, kauce wa haduwa da fata da idanu. Idan ana hulɗar haɗari, kurkura nan da nan da ruwa mai yawa kuma ku nemi taimakon likita. Tabbatar cewa an yi amfani da shi a cikin yanayin dakin gwaje-gwaje mai aminci kuma a adana shi yadda ya kamata don hana haɗari.